Difference between revisions of "Part:BBa K861169"

Latest revision as of 19:09, 26 September 2012

Indirect regulatory device, activated at high glucose concentration

This is an indirect regulatory devices activated at high glucose concentration through an intermediate protein cI of lambda phage. In this pathway, protein cI will be expressed when CRP is activated at low glucose concentration. cI will therefore repress the expression of gene downstream the promoter BBa_R0051 heavily. On the contrary, when glucose concentration is high, the promoter will be derepressed. There was no promoter existing in the nature which is directly activated by glucose. In this device, RFP was designed as a reporter. RFP can be placed by an arbitrary protein that needs to be activated by glucose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1673
Illegal AgeI site found at 1785
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K861169 short</partinfo>
-This is an indirect regulatory devices activated at high glucose concentration through an intermediate protein cI of lambda phage. In this pathway, protein cI will be expressed when CRP is activated at low glucose concentration. cI will therefore repress the expression of gene downstream the promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_K861169 BBa_R0051] heavily. On the contrary, when glucose concentration is high, the promoter will be derepressed.
+This is an indirect regulatory devices activated at high glucose concentration through an intermediate protein cI of lambda phage. In this pathway, protein cI will be expressed when CRP is activated at low glucose concentration. cI will therefore repress the expression of gene downstream the promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_R0051 BBa_R0051] heavily. On the contrary, when glucose concentration is high, the promoter will be derepressed.
 There was no promoter existing in the nature which is directly activated by glucose. In this device, RFP was designed as a reporter. RFP can be placed by an arbitrary protein that needs to be activated by glucose.