Difference between revisions of "Part:BBa K411003:Experience"
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We improve this kind of part, embed an RNA scaffold after similar theophylline aptamer and use split GFP to test how theophylline affect the 3D structure of whole RNA.
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We improve this part, embed an RNA scaffold after the theophylline aptamer in K411001 in K411003 to become riboscaffold [https://parts.igem.org/Part:BBa_K738002 K738002], thus empower K411001 & K411003 with new application.
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We use split GFP to test how theophylline affect the 3D structure of whole RNA.
 
[http://2012.igem.org/Team:ZJU-China/project.htm ZJU-China 2012 wiki]
 
[http://2012.igem.org/Team:ZJU-China/project.htm ZJU-China 2012 wiki]

Revision as of 15:25, 10 October 2012

ZJU-China 2012 tests K411003


Riboscaffold fig 11.jpg


The 8 different concentration of theophylline comparision on part K411003 theophylline robswitch tagged with GFP. Excitation at 480nm and emission at 535nm. Up to 10mM theophylline, cells didn’t show obvious side effects and GFP production is proportioned with theophylline concentration, showing that K411003 is an effective riboswitch which can be regulated by theophylline. When theophylline concentration is beyond a certain degree (about 10 mM), it somewhat affect cell growth and GFP production.

Results show that this is an effecitve riboswitch regulated by theophylline.


We improve this part, embed an RNA scaffold after the theophylline aptamer in K411001 in K411003 to become riboscaffold K738002, thus empower K411001 & K411003 with new application. We use split GFP to test how theophylline affect the 3D structure of whole RNA. [http://2012.igem.org/Team:ZJU-China/project.htm ZJU-China 2012 wiki]