Difference between revisions of "Part:BBa K801080:Design"

(→‎Design Notes)
(→‎Design Notes)
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'''Related BioBrick:'''
 
'''Related BioBrick:'''
−
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
+
-
−
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
+
  
 
'''Cloning details:'''<br>
 
'''Cloning details:'''<br>
−
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
+
* Designed in RFC10
−
<!--*Mutation C889G to delete XbaI restriction site-->
+
−
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
+
  
 
'''Quality control measures:'''<br>
 
'''Quality control measures:'''<br>
−
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
+
* Part was totally sequenced
−
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
+
−
<!--*Part was partly sequenced/Part was totally sequenced-->
+
  
 
'''Backbone:'''<br>
 
'''Backbone:'''<br>
−
<!--*Backbone name: pSB1C3'/?backbone_name?-->
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* Backbone name: pSB1C3
−
<!--*Resistance: Amp/Cp/Kan/Tet-->
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* Resistance: Cp
−
<!--*Copynumber: low/medium/high-->
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* Copynumber: high
  
 
'''Protein coding:'''<br>
 
'''Protein coding:'''<br>
−
<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
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* Protein: Preprothaumatin [Nucleotide 1 to 708]
−
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
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* The protein encoded is posttranslationally modified: Aa 1-22 and 229-236 are split off
−
<!--*The protein encoded is posttranslationally modified by ???.-->
+
−
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
+
  
 
'''Enzymatic activity:'''
 
'''Enzymatic activity:'''
−
<!--none/EC-number ?.?.?.?-->
+
-
  
 
'''Cytotoxicity:'''<br>
 
'''Cytotoxicity:'''<br>
−
This Biobrick leads to the production of thaumatin. We investigated the toxicity of thaumatin and incubated yeast cells (strain INVSc) with 45 mM of thaumatin. We found out, that thaumatin does not show any negative effect on the growth rate of yeast cells.  
+
This Biobrick leads to the production of thaumatin. We investigated the toxicity of thaumatin and incubated yeast cells (strain INVSc) with 45 mM of thaumatin. We found, that thaumatin does not show any negative effect on the growth rate of yeast cells.  
 
<!--none/not known/cytotoxic for ''organism name''-->
 
<!--none/not known/cytotoxic for ''organism name''-->
  
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'''Intellectual property:'''
 
'''Intellectual property:'''
−
<!--Information on patent situation.-->
+
Information on patent situation: Preprothaumatin derived from ''Thaumatococcus daniellii''
 
<!--Intellectual property claims made by the authors.-->
 
<!--Intellectual property claims made by the authors.-->
  

Revision as of 22:11, 26 September 2012

Prepro-Thaumatin, yeast codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Synthesized by IDT. The part is codon optimized for yeast. 3 forbidden restriction sites have been removed.

Please note, that the pre- and pro-part are split off, thus there is no possibility to add streptags or form other recombinant proteins.


Based on: http://www.ncbi.nlm.nih.gov/nuccore/121945717



Keywords:


Abbreviations:

Design Notes

Related BioBrick: -

Cloning details:

  • Designed in RFC10

Quality control measures:

  • Part was totally sequenced

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • Protein: Preprothaumatin [Nucleotide 1 to 708]
  • The protein encoded is posttranslationally modified: Aa 1-22 and 229-236 are split off

Enzymatic activity: -

Cytotoxicity:
This Biobrick leads to the production of thaumatin. We investigated the toxicity of thaumatin and incubated yeast cells (strain INVSc) with 45 mM of thaumatin. We found, that thaumatin does not show any negative effect on the growth rate of yeast cells.

Safety notes:

Intellectual property: Information on patent situation: Preprothaumatin derived from Thaumatococcus daniellii

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: