Difference between revisions of "Part:BBa K817033:Design"
Monkey781213 (Talk | contribs) (→Design Notes) |
Monkey781213 (Talk | contribs) (→Source) |
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PfadBA-RFP | PfadBA-RFP | ||
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| + | pfad:72bps, from | ||
| + | RBS:12bps, from BBa_K093005 | ||
| + | mRFP:681bps, from BBa_K093005 | ||
===References=== | ===References=== | ||
Revision as of 17:18, 26 September 2012
PfadBA-RBS-mRFP
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 656
Illegal AgeI site found at 768 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
PfadBA-RFP contains two parts (1)fatty acid-dependent promoter(pfad) and ribosomal binding site(RBS) (2)mRFP, a red fluorescent protein. The promoter pfad acts as a switch of downstream protein expression, and is regulated by the fatty acid-dependent repressor, FadR. mRFP plays a role as a reporter that indicates the expression or efficiency of the promoter.
In the absence of fatty acids, FadR binds at a site downstream of the pfad promoter and represses transcription. When long chain fatty acids become available, they bind to FadR, and elicit a conformational change that releases the protein from DNA, thus removing the repression.
In our design, FadR is constitutively expressed and could be repressed by adding oleic acid(a long-chain fatty acid), releasing the FadR and thereby expressing mRFP.
Source
PfadBA-RFP
pfad:72bps, from RBS:12bps, from BBa_K093005 mRFP:681bps, from BBa_K093005