Difference between revisions of "Part:BBa K817033:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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PfadBA-RFP contains two parts (1)fatty acid-dependent promoter(pfad) and ribosomal binding site(RBS) (2)mRFP, a red fluorescent protein. mRFP plays a role as a reporter that indicates the function or efficiency of the promoter. The promoter pfad acts as a regulator of downstream protein expression, and is regulated by the fatty acid-dependent promoter, fadR.
+
PfadBA-RFP contains two parts (1)fatty acid-dependent promoter(pfad) and ribosomal binding site(RBS) (2)mRFP, a red fluorescent protein. mRFP plays a role as a reporter that indicates the function or efficiency of the promoter. The promoter pfad acts as a regulator of downstream protein expression, and is regulated by the fatty acid-dependent promoter, FadR.
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In the absence of fatty acids, the protein binds at a site downstream of the promoters of the fad genes and represses transcription. When long chain fatty acids become available, they bind to FadR, and elicit a conformational change that releases the protein from DNA, thus removing the repression. In our design, fadR is constitutively expressed and could be repressed by adding oleic acid(a long-chain fatty acid), thereby expressing mRFP.
+
In the absence of fatty acids, FadR binds at a site downstream of the pfad promoter and represses transcription. When long chain fatty acids become available, they bind to FadR, and elicit a conformational change that releases the protein from DNA, thus removing the repression. In our part, FadR is constitutively expressed and could be repressed by adding oleic acid(a long-chain fatty acid), releasing the FadRs and thereby expressing mRFP.
  
 
===Source===
 
===Source===

Revision as of 16:51, 26 September 2012

PfadBA-RBS-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 656
    Illegal AgeI site found at 768
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PfadBA-RFP contains two parts (1)fatty acid-dependent promoter(pfad) and ribosomal binding site(RBS) (2)mRFP, a red fluorescent protein. mRFP plays a role as a reporter that indicates the function or efficiency of the promoter. The promoter pfad acts as a regulator of downstream protein expression, and is regulated by the fatty acid-dependent promoter, FadR. In the absence of fatty acids, FadR binds at a site downstream of the pfad promoter and represses transcription. When long chain fatty acids become available, they bind to FadR, and elicit a conformational change that releases the protein from DNA, thus removing the repression. In our part, FadR is constitutively expressed and could be repressed by adding oleic acid(a long-chain fatty acid), releasing the FadRs and thereby expressing mRFP.

Source

PfadBA-RFP

References