Difference between revisions of "Part:BBa K934025"
Line 6: Line 6:
 
[[Image:Pluxtet_assay.png|thumb|center|500px|]]
 
[[Image:Pluxtet_assay.png|thumb|center|500px|]]
  
−
To characterize the function of the Lux/Tet hybrid promoter(BBa_934024), we constructed this part Plux/tet-GFP (BBa_ K934025) by inserting Plux/tet promoter in front of a GFP coding sequence. By using the reporter cell that contains Plux/tet-GFP and constitutive LuxR and TetR generator (PlacIq-LuxR-TetR), we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, 3OC6HSL and aTc (anhydrous tetracycline).
+
To characterize the function of the Lux/Tet hybrid promoter(BBa_934024), we constructed this part Plux/tet-GFP (BBa_ K934025) by inserting Plux/tet promoter in front of a GFP coding sequence. By using the reporter cell that contains Plux/tet-GFP and constitutive LuxR and TetR generator (PlacIq-LuxR-Ptrc-TetR), we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, 3OC6HSL and aTc (anhydrous tetracycline).
  
 
In the presence of both inducers, the culture showed about 500-fold higher fluorescence intensity than that in the absence of both inducers.
 
In the presence of both inducers, the culture showed about 500-fold higher fluorescence intensity than that in the absence of both inducers.

Revision as of 16:22, 26 September 2012

Plux/tet-GFP

We constructed this part by ligating Plux/tet hybrid promoter (BBa_K934024) to the upstream of promoterless GFP generator (BBa_I13504).

Pluxtet assay.png

To characterize the function of the Lux/Tet hybrid promoter(BBa_934024), we constructed this part Plux/tet-GFP (BBa_ K934025) by inserting Plux/tet promoter in front of a GFP coding sequence. By using the reporter cell that contains Plux/tet-GFP and constitutive LuxR and TetR generator (PlacIq-LuxR-Ptrc-TetR), we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, 3OC6HSL and aTc (anhydrous tetracycline).

In the presence of both inducers, the culture showed about 500-fold higher fluorescence intensity than that in the absence of both inducers.

We improved previous Plux/tet hybrid promoter (BBa_K176000).


For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 751