Difference between revisions of "Part:BBa K934022"
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This part produces LasI enzyme in the presence of LuxR-3OC6HSL complex.
 
This part produces LasI enzyme in the presence of LuxR-3OC6HSL complex.
  
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[[Image:Plux-LasI_result.png|thumb|center|500px|]]
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[[Image:Plux-LasI_result.png|thumb|center|500px|This work was done by M.M, X.T and S.L.]]
  
 
To characterize Plux-LasI (BBa_K934022), we introduced Plux-LasI (BBa_K934022) with Ptet-LuxR ([https://parts.igem.org/Part:BBa_S03119 BBa_S03119]) to ''E.coli'' as “3OC6HSL dependent 3OC12HSL producer cell”. In this ''E.coli'', constitutively expressed LuxR activates the expression of LasI in the presence of 3OC6HSL. We then introduced Ptrc-LasR  and Plas-GFP ([https://parts.igem.org/Part:BBa_K649001 BBa_K649001]) to ''E.coli'' as a “Las reporter cell”.  
 
To characterize Plux-LasI (BBa_K934022), we introduced Plux-LasI (BBa_K934022) with Ptet-LuxR ([https://parts.igem.org/Part:BBa_S03119 BBa_S03119]) to ''E.coli'' as “3OC6HSL dependent 3OC12HSL producer cell”. In this ''E.coli'', constitutively expressed LuxR activates the expression of LasI in the presence of 3OC6HSL. We then introduced Ptrc-LasR  and Plas-GFP ([https://parts.igem.org/Part:BBa_K649001 BBa_K649001]) to ''E.coli'' as a “Las reporter cell”.  
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When the supernatants of the culture of “3OC6HSL dependent 3OC12HSL producer cell” were induced to “Las reporter cell”, GFP expression in “Las reporter cell” was activated. This result shows that Plux-lasI(K934022) is functioning.
 
When the supernatants of the culture of “3OC6HSL dependent 3OC12HSL producer cell” were induced to “Las reporter cell”, GFP expression in “Las reporter cell” was activated. This result shows that Plux-lasI(K934022) is functioning.
  
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We accomplished a positive feedback system with our improved part Plux-LasI([https://parts.igem.org/Part:BBa_K934012 BBa_K934012]).  
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'''We accomplished a positive feedback system with our improved part Plux-LasI([https://parts.igem.org/Part:BBa_K934012 BBa_K934012]).'''
  
  

Revision as of 07:28, 1 October 2012

Plux-LasI

We constructed this part by combining BBa_R0062 and BBa_K081016. This part produces LasI enzyme in the presence of LuxR-3OC6HSL complex.

This work was done by M.M, X.T and S.L.

To characterize Plux-LasI (BBa_K934022), we introduced Plux-LasI (BBa_K934022) with Ptet-LuxR (BBa_S03119) to E.coli as “3OC6HSL dependent 3OC12HSL producer cell”. In this E.coli, constitutively expressed LuxR activates the expression of LasI in the presence of 3OC6HSL. We then introduced Ptrc-LasR and Plas-GFP (BBa_K649001) to E.coli as a “Las reporter cell”.

When the supernatants of the culture of “3OC6HSL dependent 3OC12HSL producer cell” were induced to “Las reporter cell”, GFP expression in “Las reporter cell” was activated. This result shows that Plux-lasI(K934022) is functioning.

We accomplished a positive feedback system with our improved part Plux-LasI(BBa_K934012).


For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 745
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 307
  • 1000
    COMPATIBLE WITH RFC[1000]