Difference between revisions of "Part:BBa K883161"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | [[Image:CCMVNEG.png]] | + | [[Image:CCMVNEG.png EM picture of a CCMV VLP with an negetivly charged interior]] |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 14:10, 26 September 2012
CCMV_Negative core antigen with IPTG induced promotor
This construct produces CCMV core antigen subunits. The subunits have been modified in such a way that they will fold into CCMV Virus-Like Particles that have a negative interior. This was done by replacing 6 Arginine and 3 Lysine residues at the N-Terminus with Glutamic acid residues. The promotor and RBS that have been added to the gene allow induced expression of the subunits which increases the yield. The full protocol for this construct can be found on the [http://2012.igem.org/Team:Wageningen_UR Wageningen_UR 2012 Wiki].
Usage and Biology
File:CCMVNEG.png EM picture of a CCMV VLP with an negetivly charged interior
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 370
- 1000COMPATIBLE WITH RFC[1000]
*Safety notice*
This part is isolated from a virus and, when assembled, will form particles that very much resemble this virus in size and shape. However, no additional viral information is stored in this part and viral infection and/or replication can therefore be ruled out. It is completely safe for use in normal laboratory circumstances.