Difference between revisions of "Part:BBa K883161"
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The full protocol for this construct can be found on the [http://2012.igem.org/Team:Wageningen_UR Wageningen_UR 2012 Wiki].
 
The full protocol for this construct can be found on the [http://2012.igem.org/Team:Wageningen_UR Wageningen_UR 2012 Wiki].
  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
  
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[[Image:CCMVNEG.jpg]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K883161 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K883161 SequenceAndFeatures</partinfo>

Revision as of 14:08, 26 September 2012

CCMV_Negative core antigen with IPTG induced promotor

This construct produces CCMV core antigen subunits. The subunits have been modified in such a way that they will fold into CCMV Virus-Like Particles that have a negative interior. This was done by replacing 6 Arginine and 3 Lysine residues at the N-Terminus with Glutamic acid residues. The promotor and RBS that have been added to the gene allow induced expression of the subunits which increases the yield. The full protocol for this construct can be found on the [http://2012.igem.org/Team:Wageningen_UR Wageningen_UR 2012 Wiki].


Usage and Biology

File:CCMVNEG.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 370
  • 1000
    COMPATIBLE WITH RFC[1000]


*Safety notice*

This part is isolated from a virus and, when assembled, will form particles that very much resemble this virus in size and shape. However, no additional viral information is stored in this part and viral infection and/or replication can therefore be ruled out. It is completely safe for use in normal laboratory circumstances.