Difference between revisions of "Part:BBa K733001"

Revision as of 13:40, 26 September 2012

Ptms - a constitutive promoter in E. coli and B. subtilis.

Ptms is a constitutive promoter that can be recognized by SigA factor in Bacillus subtilis. Due to E. coli’s promiscuousness in recognizing transcription and translation signals, Ptms is also functional in E. coli. According to some experiments, Ptms shows quite low efficiency of transcription in B. subtilis. ( Moran et al., 1982).

For the characterization of Ptms, please refer to: BBa_K733009

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Reference

Moran, C., Lang, N., LeGrice, S., Lee, G., Stephens, M., Sonenshein, A., et al. (1982). Nucleotide sequences that signal the initiation of transcription and translation inBacillus subtilis..Molecular and General Genetics MGG,186, 339-346.

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K733001 short</partinfo>
-<i>Ptms</i> is a constitutive promoter that can be recognized by SigA factor in ''Bacillus subtilis''. Due to ''E. coli''’s promiscuousness in recognizing transcription and translation signals, pTms is also functional in ''E. coli''. According to some experiments, pTms shows quite low efficiency of transcription in ''B. subtilis''. ( Moran et al., 1982).
+<i>Ptms</i> is a constitutive promoter that can be recognized by SigA factor in ''Bacillus subtilis''. Due to ''E. coli''’s promiscuousness in recognizing transcription and translation signals, <i>Ptms</i> is also functional in ''E. coli''. According to some experiments, <i>Ptms</i> shows quite low efficiency of transcription in ''B. subtilis''. ( Moran et al., 1982).
-For the characterization of pTms, please refer to: [https://parts.igem.org/Part:BBa_K733009 BBa_K733009]
+For the characterization of <i>Ptms</i>, please refer to: [https://parts.igem.org/Part:BBa_K733009 BBa_K733009]