Difference between revisions of "Part:BBa K737067"

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According to the research of it before, we camp up with a program . Keep the sequencing of Lux Box, -35box and -10box. Then put the sequencing of C3 point of OmpR promoter into -35box and -10box.
 
According to the research of it before, we camp up with a program . Keep the sequencing of Lux Box, -35box and -10box. Then put the sequencing of C3 point of OmpR promoter into -35box and -10box.
  
 +
https://static.igem.org/mediawiki/parts/a/af/Ly3.PNG
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:30, 26 September 2012

pLux OmpR hybrid promoter

In order to optimize the function of the lux promoter, We creat a hybrid promoter which combine plux with OmpR, and in this case, plux can be lead to expression by AHL and obtain by phosphorylation of OmpR protein. According to the research of it before, we camp up with a program . Keep the sequencing of Lux Box, -35box and -10box. Then put the sequencing of C3 point of OmpR promoter into -35box and -10box.

Ly3.PNG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]