Difference between revisions of "Part:BBa K782082"

Revision as of 22:37, 26 September 2012

12x[NicTAL] operator_CMV promoter_2xBFP:NLS

• DNA binding sites for individual TAL effectors are indicated with square brackets [ ].

Introduction

Our construct contain twelve binding sites for NicTAL12 (binding site's sequence) that are cloned upstream of CMV promoter. Downstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

Figure 1: Schematic representation of the construct.

Characterization

Results: Specific TAL binding sites were further characterized with other reporter constructs.

BFP was obtained from Evrogen.

References

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698

Sequence and Features