Difference between revisions of "Part:BBa K823049:Design"

(→‎References)
 
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===References===
 
===References===
 
see single BioBricks; [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads]
 
see single BioBricks; [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads]
 +
 +
[http://www.ncbi.nlm.nih.gov/pubmed/21665972 Imamura ''et la.'', 2011]

Latest revision as of 09:54, 26 September 2012

PcotYZ - cotZ (-2aa) - gfp - Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 237
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 835


Design Notes

This part is cloned in the B. subtilis pSBBs1C backbone vector for direct integration in the amyE locus of B. subtilis.


Source

genomic B. subtilis, registry (Terminator), Plasmid (pGFPamy)

References

see single BioBricks; [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins Sporobeads]

[http://www.ncbi.nlm.nih.gov/pubmed/21665972 Imamura et la., 2011]