Difference between revisions of "Part:BBa K782088"
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TAL effectors (TALEs) are able to specifically bind to the DNA by recognizing base pairs on a DNA molecule. With TAL regulators we are able to control expression of a desired gene. Efficient transcription control can be achieved by binding of multiple TAL regulators upstream of promoter.  
 
TAL effectors (TALEs) are able to specifically bind to the DNA by recognizing base pairs on a DNA molecule. With TAL regulators we are able to control expression of a desired gene. Efficient transcription control can be achieved by binding of multiple TAL regulators upstream of promoter.  
  
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Our construct contain ten binding sites while single binding site is combined from 18 base pairs specificly recognised by [https://parts.igem.org/Part:BBa_K782006 TALB].
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Our construct contain ten binding sites upstream of minimal promoter (Figure 1). Single binding site is combined from 18 base pairs specificly recognised by [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782013 TALB:VP16].
 
   
 
   
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Single binding site sequence for TALB: TCTTCCGTTTCCACATCT
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Single binding site sequence for TALB:VP16: TCTTCCGTTTCCACATCT
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Binding sites are constructed in alternating setup.
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Besides TALB binding sites construct also contain ten alternating TALC binding sites, which we didn't use, since TALC regulator wasn't working properly.
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Binding sites are constructed in alternating setup. Besides TALB binding sites construct also contain ten alternating TALC binding sites, which we didn't use, since TALC regulator wasn't working properly.
  
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[[Image:10xB-pMIN.png]]
  
 
'''Figure 1:''' Schematic representation of the construct.
 
'''Figure 1:''' Schematic representation of the construct.

Revision as of 08:37, 26 September 2012

10x[TALB] operator_minimal promoter

  • TALB and TALC labels represents TAL effector 1297 and 1259 from zebrafish experiments (Sander et al., 2011).

Introduction

TAL effectors (TALEs) are able to specifically bind to the DNA by recognizing base pairs on a DNA molecule. With TAL regulators we are able to control expression of a desired gene. Efficient transcription control can be achieved by binding of multiple TAL regulators upstream of promoter.

Our construct contain ten binding sites upstream of minimal promoter (Figure 1). Single binding site is combined from 18 base pairs specificly recognised by TALB:VP16.

Single binding site sequence for TALB:VP16: TCTTCCGTTTCCACATCT

Binding sites are constructed in alternating setup. Besides TALB binding sites construct also contain ten alternating TALC binding sites, which we didn't use, since TALC regulator wasn't working properly.

10xB-pMIN.png

Figure 1: Schematic representation of the construct.


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 394
    Illegal NgoMIV site found at 754
    Illegal AgeI site found at 259
    Illegal AgeI site found at 594
    Illegal AgeI site found at 619
    Illegal AgeI site found at 954
  • 1000
    COMPATIBLE WITH RFC[1000]