Difference between revisions of "Part:BBa K782068"
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==Characterization==
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* Binding sites for TAL effectors were ordered from IDT.
  
  
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Results: Specific TAL binding sites were further characterized with different reporter constructs.
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==Characterization==
  
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* Binding sites for TAL effectors were ordered from IDT.  
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Specific TAL binding sites were further characterized with different reporter constructs.  
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'''Results:'''
  
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K782025 BBa_K782025]
  
 
==References==
 
==References==

Revision as of 10:36, 26 September 2012

4x[NicTAL]+4x[TALD] operator

  • TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).

Introduction

Our construct contains four specific binding sites for NicTAL12 and TALD.

Single binding sequence for NicTAL12 is: TCTATCAATGATAGA

Single binding sequence for TALD is: TCGTCCAATAGCTTCTC


4xNic+4xD.png

Figure 1. Shematic representation of four consecutive specific binding sites for NicTAL12 and TALD.


  • Binding sites for TAL effectors were ordered from IDT.


Characterization

Specific TAL binding sites were further characterized with different reporter constructs.

Results:

BBa_K782025

References

Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
    Illegal AgeI site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]