Difference between revisions of "Part:BBa K769016"

Latest revision as of 07:07, 4 October 2012

Promoter(high)-RBS-lux CABFEG-DT

Promoter(high)-RBS-lux CABFEG-DT (lux operon from Photobacterium phosphoreum NCMB844)

This part is constructed from our cloned lux operon, BBa_K769010.
The luxCABEFG is missing a luxD gene. Our aim was to make the bioluminescent reaction time faster than BBa_K769011, which is a construct where the whole lux system, luxCDABEFG is expressed under an arabinose inducible promoter. This is an intermediate of such device for shortening the reaction time. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 505
Illegal NheI site found at 3847
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 4309
Illegal XhoI site found at 1659
Illegal XhoI site found at 2167
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 790
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 684
Illegal BsaI.rc site found at 1366

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K769016 short</partinfo>
-Promoter(high)-RBS-lux CABFEG-DT
+Promoter(high)-RBS-lux CABFEG-DT (lux operon from <i>Photobacterium phosphoreum</I> NCMB844)
 <BR>https://static.igem.org/mediawiki/parts/8/84/Safety.png
+<BR>
+<BR>
+<BR> This part is constructed from our cloned lux operon, BBa_K769010.
+<BR> The <i>luxCABEFG</i> is missing a <i>luxD</i> gene. Our aim was to make the bioluminescent reaction time faster than BBa_K769011, which is a construct where the whole lux system, luxCDABEFG is expressed under an arabinose inducible promoter. This is an intermediate of such device for shortening the reaction time.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===