Difference between revisions of "Part:BBa K819010"
Zhangzidong (Talk | contribs) |
Zhangzidong (Talk | contribs) |
||
| Line 7: | Line 7: | ||
<html> | <html> | ||
<img src="https://static.igem.org/mediawiki/parts/f/fb/Peking2012_part_CheZ.png" style="width:500px;margin-left:10%;"/> | <img src="https://static.igem.org/mediawiki/parts/f/fb/Peking2012_part_CheZ.png" style="width:500px;margin-left:10%;"/> | ||
| − | |||
</html> | </html> | ||
| + | <p>Figure 1. <i>E.coli</i> was grown on semi-solid medium. a)MG1655 strain with cheZ gene deleted. b)cheZ gene under weak promoter BBa_J23112 ([https://parts.igem.org/Part:BBa_K819009 BBa_K819009]). <b>c)cheZ gene under medium promoter BBa_J23113 (this part)</b> d)wild type MG1655 strain</p> | ||
<br /> | <br /> | ||
Revision as of 07:23, 26 September 2012
Medium cheZ generator
CheZ is a significant protein in E.coli chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.
CheZ was placed under a weak promoter BBa_J23113. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain.
Figure 1. E.coli was grown on semi-solid medium. a)MG1655 strain with cheZ gene deleted. b)cheZ gene under weak promoter BBa_J23112 (BBa_K819009). c)cheZ gene under medium promoter BBa_J23113 (this part) d)wild type MG1655 strain
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]