Difference between revisions of "Part:BBa K819012"

Revision as of 04:38, 26 September 2012

Luminesensor repressible LacIM generator

LacI mutant protein is placed under SulA408 promoter
SulA408 promoter (BBa_K819017) can be repressed by LexA408 protein or protein with LexA408 domain (e.g. the optimized luminesensor constructed by Peking 2012 iGEM), without disturbance from endogenous LexA protein in E.coli.
LacI mutant from BBa_K395400 can repress lac promoter. So this part can work as a connection between two systems.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 LacI mutant protein is placed under SulA408 promoter<br />
-SulA408 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K819017 BBa_K819017]) can be repressed by LexA408 protein or protein with LexA408 domain (e.g. the optimized luminesensor [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819005 BBa_K819005] constructed by Peking 2012 iGEM), without disturbance from endogenous LexA protein in <i>E.coli</i>.
+SulA408 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K819017 BBa_K819017]) can be repressed by LexA408 protein or protein with LexA408 domain (e.g. the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819005  optimized luminesensor] constructed by Peking 2012 iGEM), without disturbance from endogenous LexA protein in <i>E.coli</i>.
 <br />LacI mutant from [https://parts.igem.org/Part:BBa_K395400 BBa_K395400] can repress lac promoter. So this part can work as a connection between two systems.