Difference between revisions of "Part:BBa K863102:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The Biobrick was designed to express the cellulose binding domain of ''Cellulomonas fimi'' exoglucanase (CBDcex) under the control of a T7-promoter. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and also a short C-terminal (Glycine, Serine) linker was added. The sequence ends with the ''Age''I-restriction-site of the Freiburg-Suffix, to make in frame assembly possible. | + | The Biobrick was designed to express the cellulose binding domain of ''Cellulomonas fimi'' exoglucanase (CBDcex) under the control of a T7-promoter. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and also a short C-terminal (Glycine, Serine) linker was added. The sequence ends with the ''Age''I-restriction-site of the Freiburg-Suffix, to make in frame [https://parts.igem.org/Assembly_standard_25 Standard 25)]assembly possible. |
===Source=== | ===Source=== |
Revision as of 17:10, 26 September 2012
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 365
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Biobrick was designed to express the cellulose binding domain of Cellulomonas fimi exoglucanase (CBDcex) under the control of a T7-promoter. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and also a short C-terminal (Glycine, Serine) linker was added. The sequence ends with the AgeI-restriction-site of the Freiburg-Suffix, to make in frame Standard 25)assembly possible.
Source
Origin of the cellulose binding domain is a cloning-vector form the fermentation group of Bielefeld University.