Difference between revisions of "Part:BBa K864050:Design"
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===Source=== | ===Source=== | ||
| − | pSIM5 | + | The pSC101ts origin was cloned from the pSIM5 plasmid from Datta et al, see reference below. One point mutation was made to remove one illegal SpeI site. |
===References=== | ===References=== | ||
Revision as of 23:49, 29 September 2012
pSC101 low copy origin of replication
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2130
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This ori was created by cloning pSC101ts from pSIM5 and making a V56A mutation in repA.
Source
The pSC101ts origin was cloned from the pSIM5 plasmid from Datta et al, see reference below. One point mutation was made to remove one illegal SpeI site.