Difference between revisions of "Part:BBa K819015:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
CheZ coding sequence was placed under RecA 408 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K819002 BBa_K819002]).
+
CheZ coding sequence was placed under RecA408 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K819002 BBa_K819002]).
  
 
===Source===
 
===Source===
  
−
CheZ was extracted from E.Coli strain MG1655 by colony PCR.<br /> RecA promoter was from [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819002 BBa_K819002].
+
CheZ was extracted from E.Coli strain MG1655 by colony PCR.<br /> RecA408 promoter was from [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819002 BBa_K819002].
  
 
===References===
 
===References===

Revision as of 22:39, 25 September 2012

Luminesensor repressible RecA408 promoter+CheZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CheZ coding sequence was placed under RecA408 promoter (BBa_K819002).

Source

CheZ was extracted from E.Coli strain MG1655 by colony PCR.
RecA408 promoter was from BBa_K819002.

References