Difference between revisions of "Part:BBa K911003:Experience"
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| + | The lysine promotor is constitutively active in both e.coli and bacillus, so expression of β-galactosidase should only be affected by the rate of transcriptional attenuation by the fluoride riboswitch. | ||
To verify that this part worked before undertaking more complex assays, we decided to run an assay with X-Gal to give us some qualititative data about its function. The results of this assay are shown below: | To verify that this part worked before undertaking more complex assays, we decided to run an assay with X-Gal to give us some qualititative data about its function. The results of this assay are shown below: | ||
Revision as of 22:02, 25 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K911003
This riboswitch came to us from the Yale team in the following construct:
The lysine promotor is constitutively active in both e.coli and bacillus, so expression of β-galactosidase should only be affected by the rate of transcriptional attenuation by the fluoride riboswitch.
To verify that this part worked before undertaking more complex assays, we decided to run an assay with X-Gal to give us some qualititative data about its function. The results of this assay are shown below:
The numbers on the tops of the tubes represent the concentration (in mM) of NaF in each culture. The amount of blue is proportional to the amount of β-galactosidase activity present in the culture, which in turn is regulated by the riboswitch. This qualatative assay demonstrated that our part worked.
To get more quantatative data, we performed a Miller assay.
User Reviews
UNIQ82fd9f6d907c4f4f-partinfo-00000000-QINU UNIQ82fd9f6d907c4f4f-partinfo-00000001-QINU