Difference between revisions of "Part:BBa K782082"

Revision as of 21:56, 25 September 2012

12x[NicTAL] operator_CMV promoter_2xBFP:NLS

Introduction

Our construct contain twelve binding sites for NicTAL12 (binding site's sequence) that are cloned upstream of CMV promoter. Downstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

Figure 1: Schematic representation of the construct.

Characterization

Results:

BFP was obtained from Evrogen.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 464
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 16
Illegal AgeI site found at 248
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1139
Illegal BsaI.rc site found at 1795
Illegal BsaI.rc site found at 2500

@@ Line 5: / Line 5: @@
 ==Introduction==
-Our construct contain twelve binding sites for [https://parts.igem.org/Part:BBa_K782007 NicTAL12] ([https://parts.igem.org/Part:BBa_K782003 sequence]) that are cloned upstream of CMV promoter. Downstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
+Our construct contain twelve binding sites for [https://parts.igem.org/Part:BBa_K782007 NicTAL12] ([https://parts.igem.org/Part:BBa_K782003 binding site's sequence]) that are cloned upstream of CMV promoter. Downstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
 [[Image:Nicbfp2.png‎‎]]