Difference between revisions of "Part:BBa K801010:Design"

Revision as of 21:50, 26 September 2012

TEF2 constitutive yeast promoter

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 137
1000
COMPATIBLE WITH RFC[1000]

Extracted from genomic DNA of S. cerevisiae using PCR.

Keywords:

Abbreviations:

Design Notes

Related BioBrick:BBa_K165037

Quality control measures:
Test digestion using XbaI+ PstI-HF, SpeI-HF+ PstI-HF

Backbone:
Backbone name: pSB1C3, Resistance: Cp

Protein coding:

Enzymatic activity:

Cytotoxicity:
none

Safety notes:
none

Intellectual property:

Corresponding part author/authors:

Source

Source:

Organism:

References

Literature references:

Database references:

@@ Line 21: / Line 21: @@
 ===Design Notes===
-'''Related BioBrick:'''
+'''Related BioBrick:'''[https://parts.igem.org/wiki/index.php?title=Part:BBaBBa_K165037: BBa_K165037]
 <!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 <!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
-'''Cloning details:'''<br>
+'''Cloning details:'''<br> Designed in RFC10/RFC23
 <!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 <!--*Mutation C889G to delete XbaI restriction site-->
 <!--*Truncation upstream/downstream compared to template, ?explanation?-->
-'''Quality control measures:'''<br>
+'''Quality control measures:'''<br> Test digestion using XbaI+ PstI-HF, SpeI-HF+ PstI-HF
 <!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 <!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 <!--*Part was partly sequenced/Part was totally sequenced-->
-'''Backbone:'''<br>
+'''Backbone:'''<br> Backbone name: pSB1C3, Resistance: Cp
 <!--*Backbone name: pSB1C3'/?backbone_name?-->
 <!--*Resistance: Amp/Cp/Kan/Tet-->
@@ Line 49: / Line 49: @@
 <!--none/EC-number ?.?.?.?-->
-'''Cytotoxicity:'''<br>
+'''Cytotoxicity:'''<br> none
 <!--none/not known/cytotoxic for ''organism name''-->
-'''Safety notes:'''<br>
+'''Safety notes:'''<br> none
 <!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 <!--Known and anticipated security issues: none/other.-->