Difference between revisions of "Part:BBa K808014"
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[[Image:Aroy_gnm.png|300px|thumb|right|Figure 1. '''Gaussian network modelling of AroY'''. The supposed membrane anchor in red. For simulation parameters [[http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY click here.]]]] | [[Image:Aroy_gnm.png|300px|thumb|right|Figure 1. '''Gaussian network modelling of AroY'''. The supposed membrane anchor in red. For simulation parameters [[http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY click here.]]]] | ||
| − | ''AroY'' codes for the protocatechuate deacarboxylase from ''Klebsiella pneumonie ssp. pneumonie''. It has a molecular mass of 57 kDa and is reported to form a complex of five identical AroY subunits. | + | ''AroY'' codes for the protocatechuate deacarboxylase from ''Klebsiella pneumonie ssp. pneumonie''. It has a molecular mass of 57 kDa and is reported to form a complex of five identical AroY subunits. [http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY Gaussian network modelling] showed a flexible helix linker. This could be either a membrane anchor or responsible for the formation of a pentamer. |
| − | + | <!-- Add more about the biology of this part here--> | |
| − | <!-- Add more about the biology of this part here | + | |
===Usage and Biology=== | ===Usage and Biology=== | ||
AroY is reported to catalyze the reaction of protocatechuate (3,4-Dihydroxybenzoate) to catechol under anaerobic conditions. | AroY is reported to catalyze the reaction of protocatechuate (3,4-Dihydroxybenzoate) to catechol under anaerobic conditions. | ||
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Revision as of 21:27, 25 September 2012
AroY: Catalyzes protocatechuate to catechol
Figure 1. Gaussian network modelling of AroY. The supposed membrane anchor in red. For simulation parameters http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY click here.
AroY codes for the protocatechuate deacarboxylase from Klebsiella pneumonie ssp. pneumonie. It has a molecular mass of 57 kDa and is reported to form a complex of five identical AroY subunits. [http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY Gaussian network modelling] showed a flexible helix linker. This could be either a membrane anchor or responsible for the formation of a pentamer.
Usage and Biology
AroY is reported to catalyze the reaction of protocatechuate (3,4-Dihydroxybenzoate) to catechol under anaerobic conditions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 309
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 37