Difference between revisions of "Part:BBa K808014"
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''AroY'' codes for the protocatechuate deacarboxylase from ''Klebsiella pneumonie ssp. pneumonie''. It has a molecular mass of 57 kDa and is reported to form a complex of five identical AroY subunits.
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[[Image:Aroy_gnm.png|300px|thumb|right|Figure 1. '''Gaussian network modelling of AroY'''. The supposed membrane anchor in red. For simulation parameters [[http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY click here.]]]]
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''AroY'' codes for the protocatechuate deacarboxylase from ''Klebsiella pneumonie ssp. pneumonie''. It has a molecular mass of 57 kDa and is reported to form a complex of five identical AroY subunits. [[http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY Gaussian network modelling]] showed a flexible helix linker. This could be either a membrane anchor or responsible for the formation of a pentamer.
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===Usage and Biology===
 
===Usage and Biology===
 
AroY is reported to catalyze the reaction of protocatechuate (3,4-Dihydroxybenzoate) to catechol under anaerobic conditions.
 
AroY is reported to catalyze the reaction of protocatechuate (3,4-Dihydroxybenzoate) to catechol under anaerobic conditions.
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Revision as of 21:23, 25 September 2012

AroY: Catalyzes protocatechuate to catechol

Figure 1. Gaussian network modelling of AroY. The supposed membrane anchor in red. For simulation parameters http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY click here.

AroY codes for the protocatechuate deacarboxylase from Klebsiella pneumonie ssp. pneumonie. It has a molecular mass of 57 kDa and is reported to form a complex of five identical AroY subunits. http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY Gaussian network modelling showed a flexible helix linker. This could be either a membrane anchor or responsible for the formation of a pentamer.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 37