Difference between revisions of "Part:BBa K782073"

Revision as of 20:45, 25 September 2012

10x[TALA+TALB] operator_CMV promoter_BFP

TALA and TALA labels represents TAL effector 1257 and 1297 from zebrafish experiments (Sander et al., 2011).

Introduction

Our construct contain TALA and TALB binding sites, which are adjacent to each other. Because of steric interference two different TAL regulators are unable to bind to its binding sites at once. TAL binding sites are cloned downstream of CMV promoter. Upstream of CMV is Blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

Figure 1: Schematic representation of the construct.

Characterization

Results:

BFP was obtained from Evrogen.

Sequence and Features