Difference between revisions of "Part:BBa K929003:Design"

(→‎Design Notes)
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[[Image:UP12_Designinfo_BBa_K929003.png|800px]] <br>
 
[[Image:UP12_Designinfo_BBa_K929003.png|800px]] <br>
  
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This part was made by cloning via RFC 10 cloning standard. The parts [https://parts.igem.org/Part:BBa_K929004 BBa_K929004]; [https://parts.igem.org/Part:BBa_I712004 BBa_I712004]; [https://parts.igem.org/Part:BBa_K404108 BBa_K404108] were serial cloned together.
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This part was made by cloning via RFC 10 cloning standard. The parts "modified AID+eGFP" ([https://parts.igem.org/Part:BBa_K929004 BBa_K929004]);"CMV" ([https://parts.igem.org/Part:BBa_I712004 BBa_I712004])and hGH polyadnylation sequence  ([https://parts.igem.org/Part:BBa_K404108 BBa_K404108]) were serial cloned together. For information how "modified AID+eGFP" ([https://parts.igem.org/Part:BBa_K929004 BBa_K929004]) was designed see its DesignPage.
  
 
===Source===
 
===Source===

Revision as of 19:07, 25 September 2012

modified AID with CMV, hGH-polyA and eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1958
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2360
    Illegal BsaI.rc site found at 770
    Illegal BsaI.rc site found at 1887
    Illegal SapI site found at 871


Design Notes

UP12 Designinfo BBa K929003.png

This part was made by cloning via RFC 10 cloning standard. The parts "modified AID+eGFP" (BBa_K929004);"CMV" (BBa_I712004)and hGH polyadnylation sequence (BBa_K404108) were serial cloned together. For information how "modified AID+eGFP" (BBa_K929004) was designed see its DesignPage.

Source

4 parts: CMV promoter (BBa_I712004), superAID(BBa_K929001), eGFP (BBa_K404316) and hGH polyadenylation signal sequence (BBa_K404108)

References