Difference between revisions of "Part:BBa K929003:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | [[Image:UP12_Designinfo_BBa_K929003.png|800px]] | + | [[Image:UP12_Designinfo_BBa_K929003.png|800px]] <br> |
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| + | This part was made by cloning via RFC 10 cloning standard. The parts [https://parts.igem.org/Part:BBa_K929004 BBa_K929004]; [https://parts.igem.org/Part:BBa_I712004 BBa_I712004]; [https://parts.igem.org/Part:BBa_K404108 BBa_K404108] were serial cloned together. | ||
===Source=== | ===Source=== | ||
Revision as of 19:04, 25 September 2012
modified AID with CMV, hGH-polyA and eGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1958
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2360
Illegal BsaI.rc site found at 770
Illegal BsaI.rc site found at 1887
Illegal SapI site found at 871
Design Notes
This part was made by cloning via RFC 10 cloning standard. The parts BBa_K929004; BBa_I712004; BBa_K404108 were serial cloned together.
Source
4 parts: CMV promoter (BBa_I712004), superAID(BBa_K929001), eGFP (BBa_K404316) and hGH polyadenylation signal sequence (BBa_K404108)