Difference between revisions of "Part:BBa K934022:Experience"
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[[Image:Plux-LasI_result.png|thumb|center|500px|]] | [[Image:Plux-LasI_result.png|thumb|center|500px|]] | ||
| − | To characterize Plux-LasI(BBa_K934022), we introduced Plux-LasI(BBa_K934022) with Ptet-LuxR(BBa_S03119) to E.coli as “3OC6HSL dependent 3OC12HSL producer cell”. In this E.coli, constitutively expressed LuxR activates the expression of LasI in the presence of 3OC6HSL. We then introduced Ptrc-LasR(BBa_K934011) and Plas-GFP(BBa_K649001) to E.coli as a “Las reporter cell”. | + | To characterize Plux-LasI(BBa_K934022), we introduced Plux-LasI([https://parts.igem.org/Part:BBa_K934022 BBa_K934022]) with Ptet-LuxR([https://parts.igem.org/Part:BBa_S03119 BBa_S03119]) to E.coli as “3OC6HSL dependent 3OC12HSL producer cell”. In this E.coli, constitutively expressed LuxR activates the expression of LasI in the presence of 3OC6HSL. We then introduced Ptrc-LasR([https://parts.igem.org/Part:BBa_K934011 BBa_K934011]) and Plas-GFP([https://parts.igem.org/Part:BBa_K649001 BBa_K649001) to E.coli as a “Las reporter cell”. |
| − | In the presence of 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, “Las reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Las reporter cell” is dually regulated by 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, this result shows that Plux-lasI(BBa_K934022) synthesized 3OC12HSL. | + | In the presence of 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, “Las reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Las reporter cell” is dually regulated by 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, this result shows that Plux-lasI([https://parts.igem.org/Part:BBa_K934022 BBa_K934022]) synthesized 3OC12HSL. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 18:58, 25 September 2012
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how you used this part and how it worked out.
Applications of BBa_K934022
To characterize Plux-LasI(BBa_K934022), we introduced Plux-LasI(BBa_K934022) with Ptet-LuxR(BBa_S03119) to E.coli as “3OC6HSL dependent 3OC12HSL producer cell”. In this E.coli, constitutively expressed LuxR activates the expression of LasI in the presence of 3OC6HSL. We then introduced Ptrc-LasR(BBa_K934011) and Plas-GFP([https://parts.igem.org/Part:BBa_K649001 BBa_K649001) to E.coli as a “Las reporter cell”.
In the presence of 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, “Las reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Las reporter cell” is dually regulated by 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, this result shows that Plux-lasI(BBa_K934022) synthesized 3OC12HSL.
User Reviews
UNIQ7c47a7243ac9bb88-partinfo-00000000-QINU UNIQ7c47a7243ac9bb88-partinfo-00000001-QINU