Difference between revisions of "Part:BBa K741006:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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It is the fusion protein of Cro and GFP with the RBS and the terminator
+
We construct crogfp by overlap PCR. The primers we design can combine cro and gfp with a linker [GGSG (BBa_K648005)] and add an RBS and a terminator to it.
  
 
===Source===
 
===Source===

Latest revision as of 01:04, 3 October 2012

RBS-crogfp-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 81
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 875


Design Notes

We construct crogfp by overlap PCR. The primers we design can combine cro and gfp with a linker [GGSG (BBa_K648005)] and add an RBS and a terminator to it.

Source

BBa_B0034 BBa_K741005 BBa_B0015

References