Difference between revisions of "Part:BBa K741003:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
Express GFP only if protein CI activated the modified promoter pRM.
+
 
 +
We think we improved BBa_I12007 (RBS-GFP-Terminator) since it can be used to test the basic level of expression of the modified pRM. If CI dimer exist, it will be activated and GFP expresses. It's relatively difficult to be repressed by Cro dimer.
  
 
===Source===
 
===Source===

Latest revision as of 19:36, 2 October 2012

pRM-RBS-GFP-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752


Design Notes

We think we improved BBa_I12007 (RBS-GFP-Terminator) since it can be used to test the basic level of expression of the modified pRM. If CI dimer exist, it will be activated and GFP expresses. It's relatively difficult to be repressed by Cro dimer.

Source

BBa_I13504 BBa_I12007

References