Difference between revisions of "Part:BBa K782073"

Revision as of 18:10, 25 September 2012

10x[TALA+TALB] operator_CMV promoter_BFP

Introduction

Our construct contain TALA and TALB binding sites, which are adjacent to each. For that reason two different TAL regulators are unable to bind to its binding sites at once because of steric interference. TAL binding sites are cloned downstream of CMV promoter. Upstream of CMV is Blue fluorescent protein. BFP in a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

Figure 1: Schematic representation of the construct.

Characterization

Results:

BFP was obtained from Evrogen.

Sequence and Features