Difference between revisions of "Part:BBa K934022"
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We constructed this part by combining [https://parts.igem.org/Part:BBa_R0062 BBa_R0062] and [https://parts.igem.org/Part:BBa_K081016 BBa_K081016].
 
We constructed this part by combining [https://parts.igem.org/Part:BBa_R0062 BBa_R0062] and [https://parts.igem.org/Part:BBa_K081016 BBa_K081016].
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This part starts generating LasI enzyme in the presence of LuxR-3OC6HSL complex.
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This part produces LasI enzyme in the presence of LuxR-3OC6HSL complex.
  
 
[[Image:Plux-LasI_result.png|thumb|center|500px|]]
 
[[Image:Plux-LasI_result.png|thumb|center|500px|]]

Revision as of 18:52, 25 September 2012

Plux-LasI

We constructed this part by combining BBa_R0062 and BBa_K081016. This part produces LasI enzyme in the presence of LuxR-3OC6HSL complex.

Plux-LasI result.png

In the presence of 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, “Las reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Las reporter cell” is dually regulated by 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, this result shows that Plux-lasI(K934022) synthesized 3OC12HSL.

We accomplished a positive feedback system with our improved part Plux-LasI(BBa_K934012).

For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 745
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 307
  • 1000
    COMPATIBLE WITH RFC[1000]