Difference between revisions of "Part:BBa K819015"
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CheZ is a significant protein in chemotaxis pathway of <i>E.coli</i>. It can prevent the cells from tumbling around and keep the cells moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.<br /> | CheZ is a significant protein in chemotaxis pathway of <i>E.coli</i>. It can prevent the cells from tumbling around and keep the cells moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.<br /> | ||
| − | CheZ placed under RecA 408 promoter ([https://parts.igem.org/Part:BBa_K819002 BBa_K819002]constructed by Peking 2012 iGEM) can be repressed by LexA 408 protein or protein with LexA 408 domain(e.g. [https://parts.igem.org/Part:BBa_K819005 optimized luminesensor BBa_K819005] constructed by Peking 2012 iGEM team), excluding the influence of endogenous LexA, forming a orthogonal modular biobrick. | + | CheZ placed under RecA 408 promoter (see [https://parts.igem.org/Part:BBa_K819002 BBa_K819002] constructed by Peking 2012 iGEM) can be repressed by LexA 408 protein or protein with LexA 408 domain(e.g. [https://parts.igem.org/Part:BBa_K819005 optimized luminesensor BBa_K819005] constructed by Peking 2012 iGEM team), excluding the influence of endogenous LexA, forming a orthogonal modular biobrick. Thus the motility of <i>E.coli</i> can be regulated<br /><br /> |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 22:25, 25 September 2012
Luminesensor repressible RecA408 promoter+CheZ
CheZ is a significant protein in chemotaxis pathway of E.coli. It can prevent the cells from tumbling around and keep the cells moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.
CheZ placed under RecA 408 promoter (see BBa_K819002 constructed by Peking 2012 iGEM) can be repressed by LexA 408 protein or protein with LexA 408 domain(e.g. optimized luminesensor BBa_K819005 constructed by Peking 2012 iGEM team), excluding the influence of endogenous LexA, forming a orthogonal modular biobrick. Thus the motility of E.coli can be regulated
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]