Difference between revisions of "Part:BBa K782069"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K782069 short</partinfo>
 
<partinfo>BBa_K782069 short</partinfo>
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==Introduction==
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TAL effectors (TALEs) are able to specifically bind to the DNA by recognizing base pairs on a DNA molecule. With TAL regulators we are able to control expression of a desired gene. Efficient transcription control can be achieved by binding of multiple TAL regulators upstream of promoter.
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Construct contain ten binding sites while single binding site combines 18 base pairs specificly recognised by TALA.
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Single binding site sequence for TALA: TTTACTGCTGCTCCCGCT
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Binding sites are constructed in alternating setup. Besides TALA binding sites construct also contain ten alternating TALC binding sites, which we didn't use, since TALC wasn't working properly.
  
 
[[Image:A.png]]
 
[[Image:A.png]]
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'''Figure 1:''' Schematic representation of the construct.
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==Characterization==
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Construct was later used for constructing different reporter plasmids to show specific binding of TALA KRAB / VP16.
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Results:
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Revision as of 16:01, 25 September 2012

10x[TALA+TALB] operator


Introduction

TAL effectors (TALEs) are able to specifically bind to the DNA by recognizing base pairs on a DNA molecule. With TAL regulators we are able to control expression of a desired gene. Efficient transcription control can be achieved by binding of multiple TAL regulators upstream of promoter.

Construct contain ten binding sites while single binding site combines 18 base pairs specificly recognised by TALA. Single binding site sequence for TALA: TTTACTGCTGCTCCCGCT Binding sites are constructed in alternating setup. Besides TALA binding sites construct also contain ten alternating TALC binding sites, which we didn't use, since TALC wasn't working properly.

A.png

Figure 1: Schematic representation of the construct.


Characterization

Construct was later used for constructing different reporter plasmids to show specific binding of TALA KRAB / VP16. Results:



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 149
    Illegal NgoMIV site found at 514
    Illegal AgeI site found at 12
    Illegal AgeI site found at 352
    Illegal AgeI site found at 377
    Illegal AgeI site found at 717
  • 1000
    COMPATIBLE WITH RFC[1000]