Difference between revisions of "Part:BBa K808013"
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[[Image:Tph3.png.png|300px|thumb|right|Figure 1. '''Homology modelling of TphA3'''. For simulation parameters [[http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphA3 click here.]]]] | [[Image:Tph3.png.png|300px|thumb|right|Figure 1. '''Homology modelling of TphA3'''. For simulation parameters [[http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphA3 click here.]]]] | ||
| − | tphA3 is coding for the small terephthalate 1,2-dioxygenase subunit from ''Comamonas testosteroni KF-1''. TphA3 forms together with tphA1 and tphA2 the terephthalic acid 1,2-dioxigenase system (TERDOS). TERDOS catalyzes the first reaction in the terephthalic acid degradation | + | tphA3 is coding for the small terephthalate 1,2-dioxygenase subunit from ''Comamonas testosteroni KF-1''. TphA3 forms together with tphA1 and tphA2 the terephthalic acid 1,2-dioxigenase system (TERDOS). TERDOS catalyzes the first reaction in the terephthalic acid degradation |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K808013 SequenceAndFeatures</partinfo> | <partinfo>BBa_K808013 SequenceAndFeatures</partinfo> | ||
Revision as of 14:17, 25 September 2012
tphA3: Catalyzes together with tphA2 TPA to DCD
Figure 1. Homology modelling of TphA3. For simulation parameters http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphA3 click here.
tphA3 is coding for the small terephthalate 1,2-dioxygenase subunit from Comamonas testosteroni KF-1. TphA3 forms together with tphA1 and tphA2 the terephthalic acid 1,2-dioxigenase system (TERDOS). TERDOS catalyzes the first reaction in the terephthalic acid degradation
Usage and Biology
The monomer of TphA3 consists of 155 amino acids a molar mass of 17.36 kDa.By gel permeation chromatography, we could show that TphA3 has a homotrimeric structure and a mass of 52.08 kDa.
Figure 2. GPC analysis of TphA3. The Peak of TphA3 has a retention time of 33 minutes. This retention time equates a molar mass of 52 kDa, approximately.http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphA3 click here.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]