Difference between revisions of "Part:BBa K883700:Design"

 
Line 17: Line 17:
 
|translated   
 
|translated   
 
|-
 
|-
|E-coil (present in the brick)
+
|K-coil (counterpart)
 
|aagatagcggcgttgaaggagaaaatcgcagcactaaaagaaaagatagcggcgttgaaggag     
 
|aagatagcggcgttgaaggagaaaatcgcagcactaaaagaaaagatagcggcgttgaaggag     
 
|KIAALKEKIAALKEKIAALKE       
 
|KIAALKEKIAALKEKIAALKE       
 
|-
 
|-
|K-coil (counterpart)       
+
|E-coil (present in the brick)       
 
|gaaattgcggcgctggaaaaagaaattgcggcgctggaaaaagaaattgcggcgctggaaaaa       
 
|gaaattgcggcgctggaaaaagaaattgcggcgctggaaaaagaaattgcggcgctggaaaaa       
 
|EIAALEKEIAALEKEIAALEK       
 
|EIAALEKEIAALEKEIAALEK       

Latest revision as of 15:23, 25 September 2012

GFP fused to a coiled coil


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 710


Design Notes

To be able to use the device, the counterpart of the E-coil needs to be fused to the protein that needs to be detected.


name sequence translated
K-coil (counterpart) aagatagcggcgttgaaggagaaaatcgcagcactaaaagaaaagatagcggcgttgaaggag KIAALKEKIAALKEKIAALKE
E-coil (present in the brick) gaaattgcggcgctggaaaaagaaattgcggcgctggaaaaagaaattgcggcgctggaaaaa EIAALEKEIAALEKEIAALEK

Source

The biobrick BBa_I13522 was used as a template for the mutations

References