Difference between revisions of "Part:BBa K784043:Design"

(New page: __NOTOC__ <partinfo>BBa_K784043 short</partinfo> <partinfo>BBa_K784043 SequenceAndFeatures</partinfo> ===Design Notes=== The cloning of this part included adding a restriction site at t...)
 
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===Design Notes===
 
===Design Notes===
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The cloning of this part included adding a restriction site at the beginning of the mutant T7 RNAP: XbaI at the 5' end, which was added by PCR (this was done to suit the RNAP for the standards). There wasn’t any necessity to add the suffix restriction sites at the 3' because it was already at the end of the RNAP.
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No design notes.
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===Source===
 
===Source===

Latest revision as of 21:13, 25 September 2012

Mutant T7 RNAP - T7*


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No design notes.

Source

Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9.

References