Difference between revisions of "Part:BBa K808013"
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[[Image:Tph3.png.png|300px|thumb|right|Figure 1. '''Homology modelling of TphA3'''. For simulation parameters [[http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphA3 click here.]]]]
 
[[Image:Tph3.png.png|300px|thumb|right|Figure 1. '''Homology modelling of TphA3'''. For simulation parameters [[http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphA3 click here.]]]]
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tphA3 is coding for the small terephthalate 1,2-dioxygenase subunit from ''Comamonas testosteroni KF-1''. TphA3 forms together with tphA1 and tphA2 the terephthalic acid 1,2-dioxigenase system (TERDOS). TERDOS catalyzes the first reaction in the terephthalic acid degradation.
 
tphA3 is coding for the small terephthalate 1,2-dioxygenase subunit from ''Comamonas testosteroni KF-1''. TphA3 forms together with tphA1 and tphA2 the terephthalic acid 1,2-dioxigenase system (TERDOS). TERDOS catalyzes the first reaction in the terephthalic acid degradation.
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===Usage and Biology===
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The monomer of TphA3 consists of 155 amino acids a molar mass of 17.36 kDa.By gel permeation chromatography, we could show that TphA3 has a homotrimeric structure and a mass of 52.08 kDa.
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[[Image:GPC_tphA3.jpg|550px|thumb|left|Figure 2. '''GPC analysis of TphA3'''. The Peak of TphA3 has a retention time of 33 minutes. This retention time equates a molar mass of 52 kDa, approximately.[[http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphA3 click here.]]]]
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Revision as of 14:13, 25 September 2012

tphA3: Catalyzes together with tphA2 TPA to DCD

Figure 1. Homology modelling of TphA3. For simulation parameters http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphA3 click here.

tphA3 is coding for the small terephthalate 1,2-dioxygenase subunit from Comamonas testosteroni KF-1. TphA3 forms together with tphA1 and tphA2 the terephthalic acid 1,2-dioxigenase system (TERDOS). TERDOS catalyzes the first reaction in the terephthalic acid degradation.

Usage and Biology

The monomer of TphA3 consists of 155 amino acids a molar mass of 17.36 kDa.By gel permeation chromatography, we could show that TphA3 has a homotrimeric structure and a mass of 52.08 kDa.

Figure 2. GPC analysis of TphA3. The Peak of TphA3 has a retention time of 33 minutes. This retention time equates a molar mass of 52 kDa, approximately.http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphA3 click here.


















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]