Difference between revisions of "Part:BBa K325909:Experience"

Revision as of 11:56, 25 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K325909

[http://2012.igem.org/Team:Peking Peking 2012 iGEM team]

We successfully constructed light communication system using this part-luxbrick, and we made supplementary characterization of it. See our project of light communication, please click [http://2012.igem.org/Team:Peking/Project/Communication here]

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User Reviews

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[http://2012.igem.org/Team:Fudan_Lux 2012iGEM team Fudan_Lux]

Growth curve of K325909

In 2012 iGEM competition we measured growth curve of K325909 in a E.coli strain dH5α.

The curve shows that induced by the arabinose, the bacteria growth

Check the RAW data of measurement.

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[http://2012.igem.org/Team:Peking/Project/Communication Peking 2012 iGEM team]

We successfully constructed light communication system using this part-luxbrick, and we made supplementary characterization of it.
Spectrum
We measured the spectrum of luminescence produced by luxbrick. As shown in the spectrum below, the luminescence has a maximum intensity at 485nm.

Figure 1. TOP10 cells harboring the luxbrick were cultured in LB medium and induced with L-arabinose at 10^-3M. 10 hours after induction, the glowing cells were measured for spectrum using SHIMADZU RF5301PC Spectrofluorophotometer. (Peking iGEM 2012)

Time course-video
We showed the time course of light emitting process of TOP10 cells transformed with luxbrick in a more visualized way, which is a short video. In the movie, the cells begin to glow 9 hours after induction, and the entire visible glowing process lasts about 10 hours. (Peking iGEM 2012)

See the video, please click [http://www.youtube.com/watch?v=fnhe1MKIO9Q&feature=youtu.be here].
(If you can't see the video above, please click [http://v.youku.com/v_show/id_XNDU0NDU0NDcy.html here])

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UNIQ21bc9b4fc8ef213d-partinfo-00000003-QINU UNIQ21bc9b4fc8ef213d-partinfo-00000004-QINU

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 <img src="https://static.igem.org/mediawiki/parts/d/de/Peking2012_part_experience_K325909_spectrum_.png" style="width:500px;margin-left:10%;"/>
-<p>Figure 1. TOP10 cells harboring the luxbrick were cultured in LB medium and induced with L-arabinose at 10<sup>-3</sup>M. 10 hours after induction, the glowing cells were measured for spectrum using SHIMADZU RF5301PC Spectrofluorophotometer. <b>Peking iGEM 2012</b></p>
+<p>Figure 1. TOP10 cells harboring the luxbrick were cultured in LB medium and induced with L-arabinose at 10<sup>-3</sup>M. 10 hours after induction, the glowing cells were measured for spectrum using SHIMADZU RF5301PC Spectrofluorophotometer. (<b>Peking iGEM 2012</b>)</p>
 </html>
-<p><br/><b>Time course-video</b><br/> We showed the time course of light emitting process of TOP10 cells transformed with luxbrick in a more visualized way, which is a short video. In the movie, the cells begin to glow 9 hours after induction, and the entire visible glowing process lasts about 10 hours. <b>Peking iGEM 2012</b> <br/><br/>See the video, please click [http://www.youtube.com/watch?v=fnhe1MKIO9Q&feature=youtu.be here].<br/>(If you can't see the video above, please click [http://v.youku.com/v_show/id_XNDU0NDU0NDcy.html here])</p>
+<p><br/><b>Time course-video</b><br/> We showed the time course of light emitting process of TOP10 cells transformed with luxbrick in a more visualized way, which is a short video. In the movie, the cells begin to glow 9 hours after induction, and the entire visible glowing process lasts about 10 hours. (<b>Peking iGEM 2012</b>)<br/><br/>See the video, please click [http://www.youtube.com/watch?v=fnhe1MKIO9Q&feature=youtu.be here].<br/>(If you can't see the video above, please click [http://v.youku.com/v_show/id_XNDU0NDU0NDcy.html here])</p>
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