Difference between revisions of "Part:BBa K784042"

Revision as of 12:29, 26 September 2012

T7*(N4) polymerase + terminator

his part includes T7*(N4) RNA Polymerase gene and double terminator (BBa_B0015). This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells.The part was made by cloning the T7*(N4) RNAP gene to the BioBrick BBa_B0015, which includes double terminator (B0010-B0012). This part is present in the plasmid pSB1AK3.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K784042 short</partinfo>
-This part has the N4 RNA Polymerase gene, RBS and terminator. This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells.
+his part includes T7*(N4) RNA Polymerase gene and double terminator (BBa_B0015). This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells.The part was made by cloning the T7*(N4) RNAP gene to the BioBrick BBa_B0015, which includes double terminator (B0010-B0012). This part is present in the plasmid pSB1AK3.