Difference between revisions of "Part:BBa K819008:Design"

(→‎Design Notes)
(→‎Source)
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
T7 promoter and LuxCDABE with their RBS are from Partsregistry.
+
lux brick (BBa_K325909) and T7 promoter (BBa_I712074) are from 2012 spring distribution kit.
  
 
===References===
 
===References===

Revision as of 08:55, 25 September 2012

Lux Operon under T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3014
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2012
    Illegal XhoI site found at 2842
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4401
    Illegal BsaI.rc site found at 1410
    Illegal SapI.rc site found at 4726


Design Notes

Lux CDABEG genes and related RBS from luxbrick(BBa_K325909) are placed under T7 promoter (BBa_K712074).

Source

lux brick (BBa_K325909) and T7 promoter (BBa_I712074) are from 2012 spring distribution kit.

References