Difference between revisions of "Part:BBa K864511"

m (Usage and Biology)
 
Line 5: Line 5:
  
 
===Usage and Biology===
 
===Usage and Biology===
The CP series of Jensen & Hammer are useful as constitutive promoters when the restriction sites included in the Anderson promoter collection are illegal.  
+
The CP series of Jensen & Hammer are useful as constitutive promoters when the restriction sites included in the Anderson promoter collection are undesirable.
  
 
===References===
 
===References===

Latest revision as of 07:54, 28 September 2012

CP44 constitutive promoter

This is a constitutive promoter characterized in E. coli and Lactococcus lactis. It has a strength of 34 (in E coli) and 12 (in L lactis) Miller units in the β-Galactosidase assay of Jensen & Hammer (1998). That makes it into a quite strong promoter in E coli and a rather weak promoter in L lactis (note that the scales are cannot be directly compared).

Usage and Biology

The CP series of Jensen & Hammer are useful as constitutive promoters when the restriction sites included in the Anderson promoter collection are undesirable.

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/] P. Jensen, K Hammer Appl: The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters Environ Microbiol. 64.1 (1998) 82–87.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]