Difference between revisions of "Part:BBa K864011"
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<partinfo>BBa_K864011 short</partinfo> | <partinfo>BBa_K864011 short</partinfo> | ||
− | pSB4S15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. | + | pSB4S15Iq is a BioBrick standard vector with low copy pSC101 replication origin <partinfo>BBa_K864050</partinfo> (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. |
This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site. | This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site. |
Revision as of 21:19, 26 September 2012
Low copy BioBrick standard vector with LacI
pSB4S15Iq is a BioBrick standard vector with low copy pSC101 replication origin BBa_K864050 (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4952
Illegal NheI site found at 2425
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4958 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4952 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 4952
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 4952
Plasmid lacks a suffix.
Illegal XbaI site found at 4967
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 2319
Illegal NgoMIV site found at 3817 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.