Difference between revisions of "Part:BBa K905000:Design"
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===Design Notes=== | ===Design Notes=== | ||
the part is designed to have a induced expression of our target protein in anaerobic enviroment. | the part is designed to have a induced expression of our target protein in anaerobic enviroment. | ||
| − | + | We use this promoter to induce the expression of GFP in ''E.coli'' in anaerobic enviroment.S. typhimurium strain VNP20009 carrying pNirBeGFP or peGFP was grown for 24 h under anaerobic or aerobic conditions. Expression of GFP was examined by fluorescence microscope. | |
| − | + | [[Image:NirB_Nanjingbio 1.png|thumb|center|480px]] | |
===Source=== | ===Source=== | ||
Revision as of 17:08, 26 September 2012
nirB promoter, it will be induced in anaerobic enviroment
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the part is designed to have a induced expression of our target protein in anaerobic enviroment. We use this promoter to induce the expression of GFP in E.coli in anaerobic enviroment.S. typhimurium strain VNP20009 carrying pNirBeGFP or peGFP was grown for 24 h under anaerobic or aerobic conditions. Expression of GFP was examined by fluorescence microscope.
Source
the source of BBa_K905000 is E.coli Top10 strain genome