Difference between revisions of "Part:BBa K747001:Design"
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<partinfo>BBa_K747001 short</partinfo> | <partinfo>BBa_K747001 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. | This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. | ||
− | The codon usage was modified to reduce homology in the sequence. A silent | + | The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene. |
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===Source=== | ===Source=== |
Revision as of 17:55, 24 September 2012
TAL-Protein_AC1_Direpeat
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 64
Design Notes
This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
Source
This Part was synthesized and flanked with BsmBI-restriction sites