Difference between revisions of "Part:BBa K929104"
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<partinfo>BBa_K929104 short</partinfo> | <partinfo>BBa_K929104 short</partinfo> | ||
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" | {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" | ||
| − | ! colspan="2" style="background:#66bbff;"| | + | ! colspan="2" style="background:#66bbff;"|Anti-GFP Nanobody |
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! colspan="2"|[[Image:UP12_BBa_K929104.png|300px]] | ! colspan="2"|[[Image:UP12_BBa_K929104.png|300px]] | ||
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<br> | <br> | ||
<br> | <br> | ||
| − | < | + | <p style="background-color: rgb(238, 221, 130); font-weight: bold;">Characterization</p> |
| + | For characterization and further applications see BBa_K929107. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 18:07, 1 October 2012
Anti-GFP Nanobody
| Anti-GFP Nanobody | |
|---|---|
| |
| BioBrick Nr. | BBa_929104 |
| RFC standard | RFC 25 |
| Requirement | pSB1C3 |
| Source | Gene synthesis by GeneArt |
| Submitted by | [http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012] |
The green fluorescent protein (GFP)-nanobody is a single-chain VHH antibody domain developed with specific binding activity against GFP and shows a Kd value of 1.4 nM. Its CDR3 loop is very short and has significantly fewer contacts with the GFP ligand compared to other nanobodies. Furthermore, the shortness of this CDR3 loop leads to the exposure of the framework 2 region, which has a major contribution to the binding with GFP.
Characterization
For characterization and further applications see BBa_K929107.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]