Difference between revisions of "Part:BBa K929004"

Revision as of 14:44, 24 September 2012

modified AID+eGFP

This is a composite part from the existing parts: modified_AID (BBa_K929001) and eGFP (BBa_K404316). Modified_AID (BBa_929001) is in RFC 10 cloning standard and has an aditional AgeI restriciton site. Therefore it can be fused with RFC 25 parts (C-terminal of modified AID), see Part_Design. This part was not used to express the fusion protein. It is an usefull intermediate that can be combined with differnt promoters and terminators. We combined this part with CMV-promoter and polyA-hGH (poly adenylation signal sequence)--> see BBa_929002.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1296
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 108
Illegal BsaI.rc site found at 1225
Illegal SapI site found at 209

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K929004 short</partinfo>
-This is a composite part from the existing parts: modified_AID ([https://parts.igem.org/Part:BBa_K929001 BBa_K929001]) and eGFP ([https://parts.igem.org/Part:BBa_K404316 BBa_K404316). Modified_AID (BBa_929001) is in RFC 10 cloning standard and has an aditional AgeI restriciton site. Therefore it can be fused with RFC 25 parts (C-terminal of modified AID), see [https://parts.igem.org/Part:BBa_K929004:Design Part_Design]. This part was '''not''' used to express the fusion protein. It is an usefull intermediate that can be combined with differnt promoters and terminators. We combined this part with CMV-promoter and polyA-hGH (poly adenylation signal sequence)--> see [https://parts.igem.org/Part:BBa_K929002 BBa_929002].
+This is a composite part from the existing parts: modified_AID ([https://parts.igem.org/Part:BBa_K929001 BBa_K929001]) and eGFP ([https://parts.igem.org/Part:BBa_K404316 BBa_K404316]). Modified_AID (BBa_929001) is in RFC 10 cloning standard and has an aditional AgeI restriciton site. Therefore it can be fused with RFC 25 parts (C-terminal of modified AID), see [https://parts.igem.org/Part:BBa_K929004:Design Part_Design]. This part was '''not''' used to express the fusion protein. It is an usefull intermediate that can be combined with differnt promoters and terminators. We combined this part with CMV-promoter and polyA-hGH (poly adenylation signal sequence)--> see [https://parts.igem.org/Part:BBa_K929002 BBa_929002].
 <!-- Add more about the biology of this part here