Difference between revisions of "Part:BBa K801020:Design"

(→‎Design Notes)
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===Design Notes===
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To remove a naturally occuring SpeI-restriction site, the naturally occuring "A" at position 757 was changed to "T" using quickchange mutagenesis.
 
To remove a naturally occuring SpeI-restriction site, the naturally occuring "A" at position 757 was changed to "T" using quickchange mutagenesis.
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* Mazzoni, C., Santori, F., Saliola, M. & Falcone, C. (2000) ‚Molecular analysis of UASE, a cis element containing stress response elements responsible for ethanol induction of the KlADH4 gene of Kluyveromyces lactis’, Res. Microbiol. 151, 19-28. PMID: 10724480
 
* Mazzoni, C., Santori, F., Saliola, M. & Falcone, C. (2000) ‚Molecular analysis of UASE, a cis element containing stress response elements responsible for ethanol induction of the KlADH4 gene of Kluyveromyces lactis’, Res. Microbiol. 151, 19-28. PMID: 10724480
  
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===Source===
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Extracted from genomic DNA of ''K. lactis'' using PCR.
 
Extracted from genomic DNA of ''K. lactis'' using PCR.
 +
 +
<br>'''Keywords:'''
 +
<!--These keywords are necessary to find your part using a fulltext sarch.-->
 +
<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
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<br>'''Abbreviations:'''
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<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
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<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
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 +
===Design Notes===
 +
 +
'''Related BioBrick:'''
 +
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
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<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
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'''Cloning details:'''<br>
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<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
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<!--*Mutation C889G to delete XbaI restriction site-->
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<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 +
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'''Quality control measures:'''<br>
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<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
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<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
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<!--*Part was partly sequenced/Part was totally sequenced-->
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'''Backbone:'''<br>
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<!--*Backbone name: pSB1C3'/?backbone_name?-->
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<!--*Resistance: Amp/Cp/Kan/Tet-->
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<!--*Copynumber: low/medium/high-->
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 +
'''Protein coding:'''<br>
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<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
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<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
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<!--*The protein encoded is posttranslationally modified by ???.-->
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<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
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'''Enzymatic activity:'''
 +
<!--none/EC-number ?.?.?.?-->
 +
 +
'''Cytotoxicity:'''<br>
 +
<!--none/not known/cytotoxic for ''organism name''-->
 +
 +
'''Safety notes:'''<br>
 +
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 +
<!--Known and anticipated security issues: none/other.-->
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 +
'''Intellectual property:'''
 +
<!--Information on patent situation.-->
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<!--Intellectual property claims made by the authors.-->
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'''Corresponding part author/authors:'''
 +
<!--https://igem.org/User_Information.cgi?user_id=????/email-->
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===Source===
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'''Source:'''<br>
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<!--*Commercial system: plasmid name, system name, company name-->
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<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
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<!--*Preexisting BioBrick ?Bba_number?-->
 +
<!--*cDNA Clone: ?clone_name?, ?company_name?-->
 +
<!--*Synthesized by ?company_name?.-->
 +
 +
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
 +
'''Organism:'''<br>
 +
<!--*Genesequence derived from ''?organism_name?''-->
 +
<!--*Codonoptimized for ''?organism_name?''-->
 +
<!--*Designed for the following Chassis: ''?organism-name?''-->
 +
<!--*Statement about functionality in other chassis.-->
 +
  
 
===References===
 
===References===
 +
<!-- Here you find templates to insert references to literature and different databases-->
 +
 +
'''Literature references:'''<br>
 +
<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
 +
 +
'''Database references:'''<br>
 +
<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
 +
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
 +
<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
 +
<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 +
<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
 +
<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->

Revision as of 21:29, 25 September 2012

KlADH4 yeast promoter, ethanol inducible


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 753
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 590



To remove a naturally occuring SpeI-restriction site, the naturally occuring "A" at position 757 was changed to "T" using quickchange mutagenesis.

The feature annotation in the UASe region (Upstream activating sequence "ethanol") is taken from:

  • Mazzoni, C., Santori, F., Saliola, M. & Falcone, C. (2000) ‚Molecular analysis of UASE, a cis element containing stress response elements responsible for ethanol induction of the KlADH4 gene of Kluyveromyces lactis’, Res. Microbiol. 151, 19-28. PMID: 10724480


Extracted from genomic DNA of K. lactis using PCR.


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: